4000-520-616
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4000-520-616
当前位置: 首页 > 产品中心 > Recombinant_antibodies > Prosci/ABO抗体[HE-14]/33-375/100微克
商品详细Prosci/ABO抗体[HE-14]/33-375/100微克
Prosci/ABO抗体[HE-14]/33-375/100微克
Prosci/ABO抗体[HE-14]/33-375/100微克
商品编号: 33-375
品牌: prosci-inc
市场价: ¥8500.00
美元价: 5100.00
产地: 美国(厂家直采)
公司:
产品分类: 重组抗体
公司分类: Recombinant_antibodies
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Specifications

Properties

SPECIESREACTIVITY:Human
TESTEDAPPLICATIONS:Func,IF,IHC-P
APPLICATIONS:FunctionalActivity-ErythrocyteAgglutination

Immunofluorescence:0.5-1ug/ml

Immunohistochemistry(FFPE):0.5-1ug/mlfor30minatRT(1)

OptimaldilutionoftheABOantibodyshouldbedeterminedbytheresearcher.

1.Stainingofformalin-fixedtissuesrequiresboilingtissuesectionsin10mMCitratebuffer,pH6.0,for10-20minfollowedbycoolingatRTfor20minutes

USERNOTE:Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher
IMMUNOGEN:AmixtureoferythrocytesofbloodgroupA1andglycoproteinfractionisolatedfromthesalivaofsecretorswithbloodgroupAwasusedastheimmunogenfortheABOantibody.
HOSTSPECIES:Mouse
PURIFICATION:PEGprecipitation
PHYSICALSTATE:Liquid
BUFFER:PBSwith0.1mg/mlBSAand0.05%sodiumazide
CONCENTRATION:0.2mg/mL
STORAGECONDITIONS:AliquotandStoreat-20C.Avoidfreez-thawcycles.
CLONALITY:Monoclonal
ISOTYPE:IgM,kappa
CONJUGATE:Unconjugated

AdditionalInfo

ALTERNATENAMES:Histo-bloodgroupABOsystemtransferase,Fucosylglycoprotein3-alpha-galactosyltransferase,Fucosylglycoproteinalpha-N-acetylgalactosaminyltransferase,Glycoprotein-fucosylgalactosidealpha-N-acetylgalactosaminyltransferase,Glycoprotein-fucosylgalactosidealpha-galactosyltransferase,Histo-bloodgroupAtransferase,Atransferase,Histo-bloodgroupBtransferase,Btransferase,NAGAT,Fucosylglycoproteinalpha-N-acetylgalactosaminyltransferasesolubleform,ABO
OFFICIALSYMBOL:ABO
GENEID:28

Background

BACKGROUND:ThisantibodyrecognizeshumanbloodgroupA(monofucosylanddifucosylAantigenswithchaintypes1,2,3,4,5,6)andForssmannantigen.Itisalsoreactivewiththeimmuno-dominantAtrisaccharide.Bloodgroupantigenexpressioninhumancoloncancerwasstudiedbymeansoftwomonoclonalantibodiesofbroadanti-A(HE-14)andanti-type3andtype4chain-basedAandH(HE-10)specificity.Theseantigenswereprovedtore-appearintumorsofthedistalcolon,theHE-10antibodyreactingmorefrequently(9outof12samples)thanHE-14(5outof12samples)andfrequentlywithsupra-nuclearstainingofthecytoplasmprobablyinthoseplacesoftheGolgiapparatuswherecarbohydrateantigensaresynthesized.ThisstainingpatternischaracteristicofHE-10innormalcolonicmucosaaswell.WithHE-14,stainingwasoftenabsentinlessdifferentiatedtumors,whileHE-10didreactinsuchtumors.Insomecases,thesetwoantibodiesgavedifferentstainingpatternsinparallelsectionsfromthesametissuesample,primarilyatthecellularlevel.Threeoutof12casesshowedbloodgroupantigenexpressioninthemucosaofthedistalcolonadjacenttothetumoronlywhenHE-10mAbwasused.
品牌介绍
 ProSci我们是抗体开发公司中的领导者。我们的研究抗体,定制抗体开发服务和领先的研究计划可帮助科学家,实验室技术人员,研究人员和其他技术人员降低研究成本,灵活而广泛的抗体服务,在线支持以及丰富的抗体使用第一手知识因为它们与特定的免疫化学应用有关。