Prosci/ABO抗体[HE-14]/33-375/100微克
商品编号:
33-375
品牌:
prosci-inc
市场价:
¥8500.00
美元价:
5100.00
产品分类:
重组抗体
公司分类:
Recombinant_antibodies
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Specifications
SPECIESREACTIVITY: | Human |
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TESTEDAPPLICATIONS: | Func,IF,IHC-P |
APPLICATIONS: | FunctionalActivity-ErythrocyteAgglutination Immunofluorescence:0.5-1ug/ml Immunohistochemistry(FFPE):0.5-1ug/mlfor30minatRT(1) OptimaldilutionoftheABOantibodyshouldbedeterminedbytheresearcher. 1.Stainingofformalin-fixedtissuesrequiresboilingtissuesectionsin10mMCitratebuffer,pH6.0,for10-20minfollowedbycoolingatRTfor20minutes |
USERNOTE: | Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher |
IMMUNOGEN: | AmixtureoferythrocytesofbloodgroupA1andglycoproteinfractionisolatedfromthesalivaofsecretorswithbloodgroupAwasusedastheimmunogenfortheABOantibody. |
HOSTSPECIES: | Mouse |
PURIFICATION: | PEGprecipitation |
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PHYSICALSTATE: | Liquid |
BUFFER: | PBSwith0.1mg/mlBSAand0.05%sodiumazide |
CONCENTRATION: | 0.2mg/mL |
STORAGECONDITIONS: | AliquotandStoreat-20C.Avoidfreez-thawcycles. |
CLONALITY: | Monoclonal |
ISOTYPE: | IgM,kappa |
CONJUGATE: | Unconjugated |
AdditionalInfo
ALTERNATENAMES: | Histo-bloodgroupABOsystemtransferase,Fucosylglycoprotein3-alpha-galactosyltransferase,Fucosylglycoproteinalpha-N-acetylgalactosaminyltransferase,Glycoprotein-fucosylgalactosidealpha-N-acetylgalactosaminyltransferase,Glycoprotein-fucosylgalactosidealpha-galactosyltransferase,Histo-bloodgroupAtransferase,Atransferase,Histo-bloodgroupBtransferase,Btransferase,NAGAT,Fucosylglycoproteinalpha-N-acetylgalactosaminyltransferasesolubleform,ABO |
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OFFICIALSYMBOL: | ABO |
GENEID: | 28 |
Background
BACKGROUND: | ThisantibodyrecognizeshumanbloodgroupA(monofucosylanddifucosylAantigenswithchaintypes1,2,3,4,5,6)andForssmannantigen.Itisalsoreactivewiththeimmuno-dominantAtrisaccharide.Bloodgroupantigenexpressioninhumancoloncancerwasstudiedbymeansoftwomonoclonalantibodiesofbroadanti-A(HE-14)andanti-type3andtype4chain-basedAandH(HE-10)specificity.Theseantigenswereprovedtore-appearintumorsofthedistalcolon,theHE-10antibodyreactingmorefrequently(9outof12samples)thanHE-14(5outof12samples)andfrequentlywithsupra-nuclearstainingofthecytoplasmprobablyinthoseplacesoftheGolgiapparatuswherecarbohydrateantigensaresynthesized.ThisstainingpatternischaracteristicofHE-10innormalcolonicmucosaaswell.WithHE-14,stainingwasoftenabsentinlessdifferentiatedtumors,whileHE-10didreactinsuchtumors.Insomecases,thesetwoantibodiesgavedifferentstainingpatternsinparallelsectionsfromthesametissuesample,primarilyatthecellularlevel.Threeoutof12casesshowedbloodgroupantigenexpressioninthemucosaofthedistalcolonadjacenttothetumoronlywhenHE-10mAbwasused. |
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品牌介绍
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