Prosci/Aak1抗体/4831/0.02 mg
商品编号：
4831
品牌：
prosci-inc
市场价：
¥1580.00
美元价：
948.00
产品分类：
重组抗体
公司分类：
Recombinant_antibodies
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Specifications
| SPECIES REACTIVITY: | Human, Mouse, Rat |
|---|---|
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (94%) |
| TESTED APPLICATIONS: | ELISA, WB |
| APPLICATIONS: | Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 μg/mL. |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| POSITIVE CONTROL: | 1) Cat. No. 1288 - A20 Cell Lysate |
| IMMUNOGEN: | Aak1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human Aak1. The immunogen is located within amino acids 830 - 880 of Aak1. |
| HOST SPECIES: | Rabbit |
Properties
| PURIFICATION: | Aak1 Antibody is affinity chromatography purified via peptide column. |
|---|---|
| PHYSICAL STATE: | Liquid |
| BUFFER: | Aak1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | Aak1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
Additional Info
| ALTERNATE NAMES: | Aak1 Antibody: KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1 |
|---|---|
| ACCESSION NO.: | NP_055726 |
| PROTEIN GI NO.: | 148277037 |
| OFFICIAL SYMBOL: | AAK1 |
| GENE ID: | 22848 |
Background
| BACKGROUND: | Aak1 Antibody: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane. |
|---|---|
| REFERENCES: | 1) Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol.2002; 156:921-9. | 2) Smythe E and Ayscough KR. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton. EMBO Rep.2003; 4:246-51. | 3) Ricotta D, Connor SD, Schmid SL, et al. Phosphorylation of the AP2 m2 subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol.2002; 156:791-5. | 4) Connor SD and Henderson DM. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol. Biol. Cell2007; 18:2698-706. |
品牌介绍
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